ELISpot assays - dual colour now a reality

Over the 5 years that we have been working with A.I.D's ELISpot readers and ELISpot kits, lots have people have talked about the possibility of doing dual colour assays (being able to assess two proteins in one well). This was always difficult using standard chromogenic ELISpots and in our opinion the best way that this would move forward was by using fluorescently labelled antibodies. Great! Let's try it! Ahh, how are we going to read it?

And therein lay the problem, no assays and no way of reading them even if they could be made.

Now A.I.D. have made the iSpot, an ELISpot reader capable of reading and analysing immunofluorescent ELISpot assays as well as normal chrmogenic ELISpots.

Commercial availability of fluorescent ELISpot kits is still a little patchy, but at least thee technology is now available to deal with them